Quantitative data analysis methods for bead-based DNA hybridization assays using generic flow cytometry platforms
نویسندگان
چکیده
منابع مشابه
A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry.
A new multiplexed, bead-based method which utilizes nucleic acid hybridizations on the surface of microscopic polystyrene spheres to identify specific sequences in heterogeneous mixtures of DNA sequences is described. The method consists of three elements: beads (5.6-microm diameter) with oligomer capture probes attached to the surface, three fluorophores for multiplexed detection, and flow cyt...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2008
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20534